Validation of Cyclin dependent kinase inhibitor 1B knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HSHC cells were stained with Cyclin dependent kinase inhibitor 1B antibody (R020281, 1:2,000) and analyzed using BD flow cytometer.

Flow cytometric analysis of Cyclin dependent kinase inhibitor 1B expression in HAP-1 cells using Cyclin dependent kinase inhibitor 1B antibody (R020281, 1:2,000). Green, isotype control; red, Cyclin dependent kinase inhibitor 1B.

Immunocytochemical staining of HAP-1 cells with Cyclin dependent kinase inhibitor 1B antibody (R020281, 1:1,000). Nuclei were stained blue with DAPI; Cyclin dependent kinase inhibitor 1B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using Cyclin dependent kinase inhibitor 1B antibody (R020281). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Cyclin dependent kinase inhibitor 1B antibody (R020281, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:10,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using Cyclin dependent kinase inhibitor 1B antibody (R020281). Cyclin dependent kinase inhibitor 1B expression in wild type (WT) and cyclin dependent kinase inhibitor 1B shRNA knockdown (KD) HeLa cells with 30μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Cyclin dependent kinase inhibitor 1B antibody (R020281, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.