Flow cytometric analysis of p150 CAF1 expression in HepG2 cells using p150 CAF1 antibody (R020280, 1:2,000). Green, isotype control; red, p150 CAF1.

Immunocytochemical staining of HepG2 cells with p150 CAF1 antibody (R020280, 1:1,000). Nuclei were stained blue with DAPI; p150 CAF1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Midum. Scale bar: 20 μm.

Western blotting analysis using p150 CAF1 antibody (R020280). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with p150 CAF1 antibody (R020280, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using p150 CAF1 antibody (R020280). p150 CAF1 expression in wild type (WT) and p150 CAF1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with p150 CAF1 antibody (R020280, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:50,000) respectively. Image was developed using ECL Substrate Kit.