Flow cytometric analysis of CRKII expression in HepG2 cells using CRKII antibody (R020259, 1:2,000). Green, isotype control; red, CRKII.

Immunocytochemical staining of HepG2 cells with CRKII antibody (R020259, 1:1,000). Nuclei were stained blue with DAPI; CRKII was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using CRKII antibody (R020259). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CRKII antibody (R020259, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using CRKII antibody (R020259). CRKII expression in wild type (WT) and CRKII shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with CRKII antibody (R020259, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.