Flow cytometric analysis of Amyloid beta precursor protein expression in HepG2 cells using Amyloid beta precursor protein antibody (R020255, 1:2,000). Green, isotype control; red, Amyloid beta precursor protein.

Immunocytochemical staining of HT-1080 cells with Amyloid beta precursor protein antibody (R020255, 1:1,000). Nuclei were stained blue with DAPI; Amyloid beta precursor protein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using Amyloid beta precursor protein antibody (R020255). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Amyloid beta precursor protein antibody (R020255, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using Amyloid beta precursor protein antibody (R020255). Amyloid beta precursor protein expression in wild type (WT) and amyloid beta precursor protein shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Amyloid beta precursor protein antibody (R020255, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Immunocytochemical staining of HeLa cells using Amyloid beta precursor protein antibody (R020255, 1:1,000), Top panel: wild-type (WT); Bottom panal: Amyloid beta precursor protein shRNA knockdown (KD). Nuclei were stained blue with DAPI; Amyloid beta precursor protein was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

Western blotting analysis using amyloid beta precursor protein antibody (R020255). Amyloid beta precursor protein expression in wild type (WT) and amyloid beta precursor protein (APP) knockout (KO) 293T cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with amyloid beta precursor protein antibody (R020255, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.