Flow cytometric analysis of Caspase-3 expression in HT-1080 cells using Caspase-3 antibody (R020252, 1:2,000). Green, isotype control; red, Caspase-3.

Immunocytochemical staining of HT-1080 cells with Caspase-3 antibody (R020252, 1:1,000) . Nuclei were stained blue with DAPI; Caspase-3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Immunohistochemistry was performed on paraffin-embedded human tonsillitis using caspase-3 antibody (R020252, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

Western blotting analysis using Caspase 3 antibody (R020252). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Caspase 3 antibody (R020252,1:10,000) and HRP-conjugated goat anti rabbit secondary antibody (1:50,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using Caspase-3 antibody (R020252). Caspase-3 expression in wild type (WT) and Caspase-3 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Caspase-3 antibody (R020252,1:10,000) and HRP-conjugated goat anti rabbit secondary antibody (1:50,000) respectively. Image was developed using ECL Substrate Kit.