Flow cytometric analysis of STAT6 expression in HT-1080 cells using STAT6 antibody (R020246, 1:2,000). Green, isotype control; red, STAT6.

Immunocytochemical staining of HT-1080 cells with STAT6 antibody (R020246, 1:1,000). Nuclei were stained blue with DAPI; STAT6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using STAT6 antibody (R020246). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with STAT6 antibody (R020246, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using STAT6 antibody (R020246). STAT6 expression in wild-type (WT) and STAT6 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with STAT6 antibody (R020246, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.