Flow cytometric analysis of NUMB expression in HepG2 cells using NUMB antibody (R020240, 1:2,000). Green, isotype control; red, NUMB.

Immunocytochemical staining of HepG2 cells with NUMB antibody (R020240, 1:1,000). Nuclei were stained blue with DAPI; NUMB was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Meduim. Scale bar: 20 μm.

Western blotting analysis using NUMB antibody (R020240). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NUMB antibody (R020240, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:50,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using NUMB antibody (R020240). NUMB expression in wild type (WT) and NUMB shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. GAPDH serves as a loading control. The blot was incubated with NUMB antibody (R020240, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:50,000) respectively. Image was developed using ECL Substrate Kit.