Western blotting analysis using Glutathione Synthetase antibody (R020239). Glutathione Synthetase expression in wild type (WT) and Glutathione Synthetase shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Glutathione Synthetase antibody (R020239, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Validation of Glutathione synthetase knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Glutathione synthetase antibody (R020239, 1:2,000) and analyzed using BD flow cytometer.

Flow cytometric analysis of Glutathione synthetase expression in HepG2 cells using Glutathione synthetase antibody (R020239, 1:2,000). Green, isotype control; red, Glutathione synthetase.

Immunocytochemical staining of HepG2 cells with Glutathione synthetase antibody (R020239, 1:1,000). Nuclei were stained blue with DAPI; Glutathione synthetase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using Glutathione synthetase antibody (R020239). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Glutathione synthetase antibody (R020239, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.