Western blotting analysis using BCL2 like 11 antibody (R020225). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with BCL2 like 11 antibody (R020225, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using BCL2 like 11 antibody (R020225). BCL2 like 11 expression in wild type (WT) and BCL2 like 11 (BCL2L11) knockout (KO) HSHC cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with BCL2 like 11 antibody (R020225, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Flow cytometric analysis of BCL2 like 11 expression in HepG2 cells using BCL2 like 11 antibody (R020225, 1:2,000). Green, isotype control; red, BCL2 like 11.

Immunocytochemical staining of HepG2 cells with BCL2 like 11 antibody (R020225, 1:1,000). Nuclei were stained blue with DAPI; BCL2 like 11 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.