Immunohistochemistry was performed on paraffin-embedded mouse lung using caveolin 1 antibody (R020217, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

Immunohistochemistry was performed on paraffin-embedded mouse brown adipose tissue using caveolin 1 antibody (R020217, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using caveolin 1 antibody (R020217, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

Western blotting analysis using Caveolin 1 antibody (R020217). Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Caveolin 1 antibody (R020217, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using caveolin 1 antibody (R020217). Caveolin 1 expression in wild-type (WT) and caveolin 1 (CAV1) knockout (KO) 293T cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with caveolin 1 antibody (R020217, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.