Flow cytometric analysis of 14-3-3 alpha/beta expression in HepG2 cells using 14-3-3 alpha/beta antibody (R020210, 1:2,000). Green, isotype control; red, 14-3-3 alpha/beta.

Immunocytochemical staining of HepG2 cells with 14-3-3 alpha/beta antibody (R020210, 1:1,000). Nuclei were stained blue with DAPI; 14-3-3 alpha/beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using 14-3-3 alpha/beta antibody (R020210). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with 14-3-3 alpha/beta antibody (R020210, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using 14-3-3 alpha/beta antibody (R020210). 14-3-3 alpha/beta expression in wild-type (WT) and YWHAB knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with 14-3-3 alpha/beta antibody (R020210, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.