Immunocytochemical staining of HepG2 cells with SMAC antibody (R020209, 1:1,000). Nuclei were stained blue with DAPI; SMAC was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using DIABLO antibody (R020209). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DIABLO antibody (R020209, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using SMAC antibody (R020209). SMAC expression in wild-type (WT) and DIABLO knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SMAC antibody (R020209, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Flow cytometric analysis of SMAC expression in HepG2 cells using SMAC antibody (R020209, 1:2,000). Green, isotype control; red, SMAC.