Western blotting analysis using gelsolin antibody (R020082). Gelsolin expression in wild-type (WT) and gelsolin (GSN) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with gelsolin antibody (R020082, 1:5,000) and HRP-conjugated goat rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using gelsolin antibody (R020082). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with gelsolin antibody (R020082, 1:5,000) and HRP-conjugated goat rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Flow cytometric analysis of Gelsolin expression in C2C12 cells using Gelsolin antibody (R020082, 1:2,000). Green, isotype control; red, Gelsolin.

Validation of Gelsolin knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HT-1080 cells were stained with Gelsolin antibody (R020082, 1:2,000) and analyzed using BD flow cytometer.