Western blotting analysis using DNA fragmentation factor subunit alpha antibody (R020073). DNA fragmentation factor subunit alpha expression in wild type (WT) and DNA fragmentation factor subunit alpha (DFFA) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with DNA fragmentation factor subunit alpha antibody (R020073, 1:5,000) and HRP-conjugated goat rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using DNA fragmentation factor subunit alpha antibody (R020073). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with DNA fragmentation factor subunit alpha antibody (R020073, 1:5,000) and HRP-conjugated goat rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Flow cytometric analysis of DNA fragmentation factor subunit alpha expression in HepG2 cells using DNA fragmentation factor subunit alpha antibody (R020073, 1:2,000). Green, isotype control; red, DNA fragmentation factor subunit alpha.