Western blotting analysis using tissue factor pathway inhibitor antibody (R020052). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with tissue factor pathway inhibitor antibody (R020052, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Western blotting analysis using tissue factor pathway inhibitor antibody (R020052). Tissue factor pathway inhibitor expression in wild-type (WT) and tissue factor pathway inhibitor (TFP1) shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with tissue factor pathway inhibitor antibody (R020052, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using ECL Substrate Kit.

Validation of Tissue factor pathway inhibitor knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HepG2 cells were stained with Tissue factor pathway inhibitor antibody (R020052, 1:2,000) and analyzed using BD flow cytometer.