Immunofluorescence - Anti-PDCD4 Rabbit mAb [83L86M98]

Sample: HeLa cells

The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.5% Triton X-100 for 10 minutes and then blocked with 5% BSA in 0.1% PBS-Tween for 0.5 hours.

Primary antibodies: R016157 at 1:100 dilution and α-tubulin Mouse Monoclonal Antibody (Cat. No. LF209) at 1:100 dilution

Secondary antibodies: Goat anti-Rabbit (488) at 1:1,000 dilution (shown in green) and Goat anti-Mouse (555) at 1:1,000 dilution (shown in red)

Nuclei were stained with DAPI (shown in blue).

Western Blot - Anti-PDCD4 Rabbit mAb [83L86M98]

All lanes: R016157 at 1:4,000 dilution

Lane 1: Hel (Human erythroLeukemia  suspension cell) whole cell lysates

Lane 2: Ball-1 (Human B lymphocyte acute leukemia cell) whole cell lysates

Lane 3: K562 (Human chronic myeloid leukemia cell) whole cell lysates

Lane 4: THP-1 (Human monocytic leukemia cell) whole cell lysates

Lane 5: PANC-1 (Human pancreatic cancer epithelial cell) whole cell lysates

Lane 6: U87 (Human malignant glioblastoma epithelial cells) whole cell lysates

Lane 7: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 52 kDa

Observed band size: 59 kDa

Developed using the ECL technique (Cat. No. SQ201).