Western Blot - Anti-Phospho-CRK (Tyr221) Rabbit mAb [12L43F75]

All lanes: R016110 at 1:1,000 dilution

Lane 1: Untreated THP-1 (Human hepatocarcinoma epithelial cell) whole cell lysates

Lane 2: THP-1treated with 10nM insulin for 15min

Lane 3: Untreated PC-12 (Rat adrenal pheochromocytoma epithelial cell) whole cell lysates

Lane 4: PC-12 treated with 10nM insulin for 15min

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 34 kDa

Observed band size: 38 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunohistochemistry - Anti-Phospho-CRK (Tyr221) Rabbit mAb [12L43F75]

Sample: Paraformaldehyde-fixed, paraffin embedded rat kidney tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R016110 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunohistochemistry - Anti-Phospho-CRK (Tyr221) Rabbit mAb [12L43F75]

Sample: Paraformaldehyde-fixed, paraffin embedded human hepatoma tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R016110 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.