Anti-MMP21 Rabbit mAb [75B42J76]

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Anti-MMP21 Rabbit mAb [75B42J76]

Purified Recombinant Rabbit Monoclonal Antibody

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货号	规格	价格
R016096
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Western Blot - Anti-MMP21 Rabbit mAb [75B42J76]

All lanes: R016096 at 1:1,000 dilution

Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

Lane 2: HepG2 (Human hepatocarcinoma epithelial cell) whole cell lysates

Lane 3: 293T (Human embryonic kidney cell) whole cell lysates

Lane 4: K562 (Human chronic myeloid leukemia cell) whole cell lysates

Lane 5: U87 (Human malignant glioblastoma epithelial cells) whole cell lysates

Lane 6: C2C12 (Mouse myoblasts epithelial cell) whole cell lysates

Lane 7: Mouse testicular whole tissue lysates

Lane 8: Mouse Ovary whole tissue lysates

Lane 9: PC-12 (Rat adrenal pheochromocytoma epithelial cell) whole cell lysates

Lane 10: Rat testicular whole tissue lysates

Lane 11: Rat Ovary whole tissue lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 65 kDa

Observed band size: 48 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunohistochemistry - Anti-MMP21 Rabbit mAb [75B42J76]

Sample: Paraformaldehyde-fixed, paraffin embedded rat testis tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R016096 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunohistochemistry - Anti-MMP21 Rabbit mAb [75B42J76]

Sample: Paraformaldehyde-fixed, paraffin embedded human ovarian cancer tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R016096 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunohistochemistry - Anti-MMP21 Rabbit mAb [75B42J76]

Sample: Paraformaldehyde-fixed, paraffin embedded mouse ovary tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R016096 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

产品信息
说明书
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Product Name	Anti-MMP21 Rabbit mAb
Description	Purified Recombinant Rabbit Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P/IF (Tissue-P), ELISA
Dilution	WB 1:1,000~1:2,000; IHC-P 1:100~1:200; IF 1:100~1:200
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal
Clone No.	75B42J76
Isotype	IgG
Label	Unconjugated
Immunogen	A synthesized peptide derived from human MMP21
Format	Affinity purified monoclonal antibody supplied in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

Synonyms	HTX7; MMP-21; b2b2458Clo; b2b873Clo; MMP21_HUMAN; MMP21; 3.4.24.-; MMP21_MOUSE.
Molecular weight	Calculated MW: 65 kDa; Observed MW: 48 kDa
Uniprot ID	Q8N119
Gene ID	118856
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-MMP21 Rabbit mAb [75B42J76] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	This gene encodes a member of the matrix metalloproteinase family. Proteins in this family are involved in the breakdown of extracellular matrix for both normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, and disease processes, such as asthma and tumor metastasis. The encoded protein may play an important role in embryogenesis, particularly in neuronal cells, as well as in lymphocyte development and survival. [provided by RefSeq, May 2013]

Background

This gene encodes a member of the matrix metalloproteinase family. Proteins in this family are involved in the breakdown of extracellular matrix for both normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, and disease processes, such as asthma and tumor metastasis. The encoded protein may play an important role in embryogenesis, particularly in neuronal cells, as well as in lymphocyte development and survival. [provided by RefSeq, May 2013]

Cellular Location	Secreted

Tissue Location	Identified in fetal brain, kidney and liver. In adult tissues found primarily in ovary, kidney, liver, lung, placenta, brain and peripheral blood leukocytes. Expressed as well in various cancer cell lines.

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R016096 Anti-MMP21 Rabbit mAb [75B42J76]

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