Anti-SIRT7 Rabbit mAb [30N69R77]

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Anti-SIRT7 Rabbit mAb [30N69R77]

Purified Recombinant Rabbit Monoclonal Antibody

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货号	规格	价格
R016045
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Western Blot - Anti-SIRT7 Rabbit mAb [30N69R77]

All lanes: R016045 at 1:1,000 dilution

Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

Lane 2: HepG2 (Human hepatocarcinoma epithelial cell) whole cell lysates

Lane 3: HCT116 (Human colorectal carcinoma epithelial cell) whole cell lysates

Lane 4: MCF-7 (human breast adenocarcinoma epithelial cell) whole cell lysates

Lane 5: 293T (Human embryonic kidney cell) whole cell lysates

Lane 6: C2C12 (Mouse myoblasts epithelial cell) whole cell lysates

Lane 7: Mouse liver whole tissue lysates

Lane 8: PC-12 (Rat adrenal pheochromocytoma epithelial cell) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 45 kDa

Observed band size: 45 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunohistochemistry - Anti-SIRT7 Rabbit mAb [30N69R77]

Sample: Paraformaldehyde-fixed, paraffin embedded human cervical cancer tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R016045 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunohistochemistry - Anti-SIRT7 Rabbit mAb [30N69R77]

Sample: Paraformaldehyde-fixed, paraffin embedded mouse liver tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R016045 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Product Name	Anti-SIRT7 Rabbit mAb
Description	Purified Recombinant Rabbit Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P/IF (Tissue-P), ELISA
Dilution	WB 1:1,000~1:2,000; IHC-P 1:100~1:200; IF 1:100~1:200
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal
Clone No.	30N69R77
Isotype	IgG
Label	Unconjugated
Immunogen	Recombinant protein of human SIRT7
Format	Affinity purified monoclonal antibody supplied in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

Synonyms	NAD dependent deacetylase sirtuin 7; NAD-dependent deacetylase sirtuin-7; Silent mating type information regulation 2 homolog; Silent mating type information regulation 2 S.cerevisiae homolog 7; SIR2 L7; SIR2 like protein 7; Sir2 related protein type 7; SIR2, S.CEREVISIAE, HOMOLOG-LIKE 7; SIR2-like protein 7; SIR2L 7; SIR2L7; SIRT 7; SIR7_HUMAN; Sirtuin 7; Sirtuin type 7; Sirtuin7.
Molecular weight	Calculated MW: 45 kDa; Observed MW: 45 kDa
Uniprot ID	Q9NRC8 , Q8BKJ9 , B2RZ55
Gene ID	51547 , 209011 , 303745
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-SIRT7 Rabbit mAb [30N69R77] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. [provided by RefSeq, Jul 2008] Enables NAD-dependent protein-lysine depropionylase activity and histone H3K18 deacetylase activity, NAD-dependent. Involved in several processes, including chromatin remodeling; homologous chromosome pairing at meiosis; and protein deacylation. Located in chromatin and site of double-strand break. Is expressed in several structures, including 1st branchial arch maxillary component; central nervous system; eye; gonad; and pancreas. Orthologous to human SIRT7 (sirtuin 7). [provided by Alliance of Genome Resources, Jul 2025] Predicted to enable several functions, including NAD+ binding activity; NAD-dependent protein lysine deacylase activity; and chromatin binding activity. Predicted to be involved in several processes, including chromatin remodeling; protein deacylation; and regulation of macromolecule metabolic process. Predicted to be located in nuclear speck; nucleolus organizer region; and site of double-strand break. Predicted to be active in chromatin and nucleus. Orthologous to human SIRT7 (sirtuin 7). [provided by Alliance of Genome Resources, Apr 2025]

Background

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. [provided by RefSeq, Jul 2008] Enables NAD-dependent protein-lysine depropionylase activity and histone H3K18 deacetylase activity, NAD-dependent. Involved in several processes, including chromatin remodeling; homologous chromosome pairing at meiosis; and protein deacylation. Located in chromatin and site of double-strand break. Is expressed in several structures, including 1st branchial arch maxillary component; central nervous system; eye; gonad; and pancreas. Orthologous to human SIRT7 (sirtuin 7). [provided by Alliance of Genome Resources, Jul 2025] Predicted to enable several functions, including NAD+ binding activity; NAD-dependent protein lysine deacylase activity; and chromatin binding activity. Predicted to be involved in several processes, including chromatin remodeling; protein deacylation; and regulation of macromolecule metabolic process. Predicted to be located in nuclear speck; nucleolus organizer region; and site of double-strand break. Predicted to be active in chromatin and nucleus. Orthologous to human SIRT7 (sirtuin 7). [provided by Alliance of Genome Resources, Apr 2025]

Cellular Location	Nucleus Nucleolus Nucleus Nucleoplasm Chromosome Cytoplasm Mainly localizes in the nucleolus and nucleoplasm (PubMed:24207024, PubMed:28790157, PubMed:28886238, PubMed:31075303). Associated with rDNA promoter and transcribed region (PubMed:16079181, PubMed:19174463). Associated with nucleolar organizer regions during mitosis (PubMed:16079181, PubMed:19174463). In response to stress, released from nucleolus to nucleoplasm (PubMed:24207024). Associated with chromatin (PubMed:22722849). In response to DNA damage, recruited to DNA double-strand breaks (DSBs) sites (Probable) (PubMed:27436229). Located close to the nuclear membrane when in the cytoplasm (PubMed:11953824).

Cellular Location

Nucleus Nucleolus Nucleus Nucleoplasm Chromosome Cytoplasm Mainly localizes in the nucleolus and nucleoplasm (PubMed:24207024, PubMed:28790157, PubMed:28886238, PubMed:31075303). Associated with rDNA promoter and transcribed region (PubMed:16079181, PubMed:19174463). Associated with nucleolar organizer regions during mitosis (PubMed:16079181, PubMed:19174463). In response to stress, released from nucleolus to nucleoplasm (PubMed:24207024). Associated with chromatin (PubMed:22722849). In response to DNA damage, recruited to DNA double-strand breaks (DSBs) sites (Probable) (PubMed:27436229). Located close to the nuclear membrane when in the cytoplasm (PubMed:11953824).

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R016045 Anti-SIRT7 Rabbit mAb [30N69R77]

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