Western Blot - Anti-AP2B1 Rabbit mAb [61L45M14]
All lanes: R015954 at 1:1,000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2: HepG2 (Human hepatocarcinoma epithelial cell) whole cell lysates
Lane 3: HCT116 (Human colorectal carcinoma epithelial cell) whole cell lysates
Lane 4: 293T (Human embryonic kidney cell) whole cell lysates
Lane 5: K562 (Human chronic myeloid leukemia cell) whole cell lysates
Lane 6: Caco2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates
Lane 7: U87 (Human malignant glioblastoma epithelial cells) whole cell lysates
Lane 8: Raw264.7 (Mouse mononuclear macrophage leukemia cell) whole cell lysates
Lane 9: Mouse liver whole tissue lysates
Lane 10: Mouse adipose whole tissue lysates
Lane 11: Mouse embryo whole tissue lysates
Lane 12: PC-12 (Rat adrenal pheochromocytoma epithelial cell) whole cell lysates
Lane 13: Rat liver whole tissue lysates
Lane 14: Rat adipose whole tissue lysates
Lysates/proteins at 10 µg per lane.
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution
Predicted band size: 105 kDa
Observed band size: 105 kDa
Developed using the ECL technique (Cat. No. SQ201).