Western Blot - Anti-CRKL Rabbit mAb [54D60P42]

All lanes: R015932 at 1:1,000 dilution

Lane 1: HepG2 (Human hepatocarcinoma epithelial cell) whole cell lysates

Lane 2: HCT116 (Human colorectal carcinoma epithelial cell) whole cell lysates

Lane 3: K562 (Human chronic myeloid leukemia cell) whole cell lysates

Lane 4: U87 (Human malignant glioblastoma epithelial cells) whole cell lysates

Lane 5: C2C12 (Mouse myoblasts epithelial cell) whole cell lysates

Lane 6: Mouse brain whole tissue lysates

Lane 7: PC-12 (Rat adrenal pheochromocytoma epithelial cell) whole cell lysates

Lane 8: Rat brain whole tissue lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 34 kDa

Observed band size: 39 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunohistochemistry - Anti-CRKL Rabbit mAb [54D60P42]

Sample: Paraformaldehyde-fixed, paraffin embedded human colonic cancer tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R015932 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.