Anti-CD127 Rabbit mAb [70N09N49]

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Anti-CD127 Rabbit mAb [70N09N49]

Purified Recombinant Rabbit Monoclonal Antibody

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货号	规格	价格
R015912
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Western Blot - Anti-CD127 Rabbit mAb [70N09N49]

All lanes: R015912 at 1:1,000 dilution

Lane 1: K562 (Human chronic myeloid leukemia cell) whole cell lysates

Lane 2: U87 (Human malignant glioblastoma epithelial cells) whole cell lysates

Lane 3: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates

Lane 4: Raw264.7 (Mouse mononuclear macrophage leukemia cell) whole cell lysates

Lane 5: Mouse liver whole tissue lysates

Lane 6: Mouse liver whole tissue lysates (LPS stimulated)

Lane 7: PC-12 (Rat adrenal pheochromocytoma epithelial cell) whole cell lysates

Lane 8: Rat liver whole tissue lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 52 kDa

Observed band size: 75 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunohistochemistry - Anti-CD127 Rabbit mAb [70N09N49]

Sample: Paraformaldehyde-fixed, paraffin embedded human colonic cancer tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R015912 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunohistochemistry - Anti-CD127 Rabbit mAb [70N09N49]

Sample: Paraformaldehyde-fixed, paraffin embedded mouse spleen tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R015912 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

产品信息
说明书
已发表文献

Product Name	Anti-CD127 Rabbit mAb
Description	Purified Recombinant Rabbit Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P/IF (Tissue-P), ELISA
Dilution	WB 1:1,000~1:2,000; IHC-P 1:100~1:200; IF 1:100~1:200
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal
Clone No.	70N09N49
Isotype	IgG
Label	Unconjugated
Immunogen	A synthesized peptide derived from human IL7R alpha/CD127
Format	Affinity purified monoclonal antibody supplied in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

Synonyms	CD127; Interleukin-7 receptor subunit alpha; IL-7 receptor subunit alpha; IL-7R subunit alpha; IL-7R-alpha; IL-7RA; CDw127; IL7R.
Molecular weight	Calculated MW: 52 kDa; Observed MW: 75 kDa
Uniprot ID	P16871
Gene ID	3575
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-CD127 Rabbit mAb [70N09N49] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	The protein encoded by this gene is a receptor for interleukin 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukins 2, 4, 7, 9, and 15. This protein has been shown to play a critical role in V(D)J recombination during lymphocyte development. Defects in this gene may be associated with severe combined immunodeficiency (SCID). Alternatively spliced transcript variants have been found. [provided by RefSeq, Dec 2015]

Background

The protein encoded by this gene is a receptor for interleukin 7 (IL7). The function of this receptor requires the interleukin 2 receptor, gamma chain (IL2RG), which is a common gamma chain shared by the receptors of various cytokines, including interleukins 2, 4, 7, 9, and 15. This protein has been shown to play a critical role in V(D)J recombination during lymphocyte development. Defects in this gene may be associated with severe combined immunodeficiency (SCID). Alternatively spliced transcript variants have been found. [provided by RefSeq, Dec 2015]

Cellular Location	Isoform 1.Cell membrane.Single-pass type I membrane protein.Isoform 3.Cell membrane.Single-pass type I membrane protein.Isoform 4.Secreted.

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R015912 Anti-CD127 Rabbit mAb [70N09N49]

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