Anti-MEK3/MEK6 Rabbit mAb [94C58M33]

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Anti-MEK3/MEK6 Rabbit mAb [94C58M33]

Purified Recombinant Rabbit Monoclonal Antibody

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货号	规格	价格
R015759
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Western Blot - Anti-MEK3/MEK6 Rabbit mAb [94C58M33]

All lanes: R015759 at 1:1,000 dilution

Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

Lane 2: HepG2 (Human hepatocarcinoma epithelial cell) whole cell lysates

Lane 3: HCT116 (Human colorectal carcinoma epithelial cell) whole cell lysates

Lane 4: 293T (Human embryonic kidney cell) whole cell lysates

Lane 5: K562 (Human chronic myeloid leukemia cell) whole cell lysates

Lane 6: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates

Lane 7: C2C12 (Mouse myoblasts epithelial cell) whole cell lysates

Lane 8: Mouse heart whole tissue lysates

Lane 9: Mouse brain whole tissue lysates

Lane 10: PC-12 (Rat adrenal pheochromocytoma epithelial cell) whole cell lysates

Lane 11: Rat liver whole tissue lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 39,37 kDa

Observed band size: 39,37 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunohistochemistry - Anti-MEK3/MEK6 Rabbit mAb [94C58M33]

Sample: Paraformaldehyde-fixed, paraffin embedded human lung cancer tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R015759 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunofluorescence - Anti-MEK3/MEK6 Rabbit mAb [94C58M33]

Sample: HeLa cells

The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.5% Triton X-100 for 10 minutes and then blocked with 5% BSA in 0.1% PBS-Tween for 0.5 hours.

Primary antibodies: R015759 at 1:100 dilution and α-tubulin Mouse Monoclonal Antibody (Cat. No. LF209) at 1:100 dilution

Secondary antibodies: Goat anti-Rabbit (488) at 1:1,000 dilution (shown in green) and Goat anti-Mouse (555) at 1:1,000 dilution (shown in red)

Nuclei were stained with DAPI (shown in blue).

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Product Name	Anti-MEK3/MEK6 Rabbit mAb
Description	Purified Recombinant Rabbit Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P/IF (Tissue-P), IF (Cell)/ICC, ELISA
Dilution	WB 1:1,000~1:2,000; IHC-P 1:100~1:200; IF 1:100~1:200
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal
Clone No.	94C58M33
Isotype	IgG
Label	Unconjugated
Immunogen	A synthesized peptide derived from human MEK3/MEK6
Format	Affinity purified monoclonal antibody supplied in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

Synonyms	Dual specificity mitogen activated protein kinase kinase 6; Dual specificity mitogen-activated protein kinase kinase 3; MAP kinase kinase 3; MAP kinase kinase 6; map2k3; MAP2K6; MAPK/ERK kinase 3; MAPK/ERK kinase 6; MAPKK 3; MAPKK 6; MAPKK3; MAPKK6; MEK 3; MEK 6; Mitogen activated protein kinase kinase 3; Mitogen activated protein kinase kinase 6; MKK3; MKK6; MP2K3_HUMAN; PRKMK3; PRKMK6; Protein kinase, mitogen activated, kinase 6 (MAP kinase kinase 6); SAPK kinase 2; SAPKK 3; SAPKK-2; SAPKK2; SAPKK3; Stress activated protein kinase kinase 3; Stress-activated protein kinase kinase 2.
Molecular weight	Calculated MW: 39,37 kDa; Observed MW: 39,37 kDa
Uniprot ID	P46734 , P52564
Gene ID	5606 , 5608
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-MEK3/MEK6 Rabbit mAb [94C58M33] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008]

Background

The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008]

Tissue Location	Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues.

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R015759 Anti-MEK3/MEK6 Rabbit mAb [94C58M33]

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