Western Blot - Anti-IRS2 Rabbit mAb [57F11H86]

All lanes: R015279 at 1:1,000 dilution

Lane 1: A549 (Human lung carinoma epithelial cell) whole cell lysates

Lane 2: A431 (Human epidermoid teratoma cell line) whole cell lysates

Lane 3: 293T (Human embryonic kidney cell) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 137 kDa

Observed band size: 185 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunohistochemistry - Anti-IRS2 Rabbit mAb [57F11H86]

Sample: Paraformaldehyde-fixed, paraffin embedded human breast cancer tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R015279 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunohistochemistry - Anti-IRS2 Rabbit mAb [57F11H86]

Sample: Paraformaldehyde-fixed, paraffin embedded mouse muscle tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R015279 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.