Anti-Beta Arrestin 2 Rabbit mAb [87J31E91]

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Anti-Beta Arrestin 2 Rabbit mAb [87J31E91]

Purified Recombinant Rabbit Monoclonal Antibody

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货号	规格	价格
R015220
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Immunohistochemistry - Anti-Beta Arrestin 2 Rabbit mAb [87J31E91]

Sample: Paraformaldehyde-fixed, paraffin embedded rat testis tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R015220 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunohistochemistry - Anti-Beta Arrestin 2 Rabbit mAb [87J31E91]

Sample: Paraformaldehyde-fixed, paraffin embedded human cervical cancer tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R015220 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunohistochemistry - Anti-Beta Arrestin 2 Rabbit mAb [87J31E91]

Sample: Paraformaldehyde-fixed, paraffin embedded mouse lung tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R015220 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Western Blot - Anti-Beta Arrestin 2 Rabbit mAb [87J31E91]

All lanes: R015220 at 1:1,000 dilution

Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

Lane 2: Huh1 (Human hepatocarcinoma epithelial cell) whole cell lysates

Lane 3: HepG2 (Human hepatocarcinoma epithelial cell) whole cell lysates

Lane 4: T24 (Human bladder cancer epithelial cell) whole cell lysates

Lane 5: U87 (Human malignant glioblastoma epithelial cells) whole cell lysates

Lane 6: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates

Lane 7: Raw264.7 (Mouse mononuclear macrophage leukemia cell) whole cell lysates

Lane 8: Mouse liver whole tissue lysates

Lane 9: PC-12 (Rat adrenal pheochromocytoma epithelial cell) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 46 kDa

Observed band size: 50 kDa

Developed using the ECL technique (Cat. No. SQ201).

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说明书
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Product Name	Anti-Beta Arrestin 2 Rabbit mAb
Description	Purified Recombinant Rabbit Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P/IF (Tissue-P), ELISA
Dilution	WB 1:1,000~1:2,000; IHC-P 1:100~1:200; IF 1:100~1:200
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal
Clone No.	87J31E91
Isotype	IgG
Label	Unconjugated
Immunogen	A synthesized peptide derived from human Beta Arrestin 2
Format	Affinity purified monoclonal antibody supplied in PBS with 0.01% sodium azide and 50% glycerol, pH 7.3.

Synonyms	ARB 2; ARB2; ARR 2; ARR2; ARRB 2; ARRB2; ARRB2_HUMAN; Arrestin 3; Arrestin beta 2; Arrestin beta-2; BARR2; Beta-arrestin-2; DKFZp686L0365; HGNC:712; Beta Arrestin 2.
Molecular weight	Calculated MW: 46 kDa; Observed MW: 50 kDa
Uniprot ID	P32121 , Q91YI4 , P29067
Gene ID	409 , 216869 , 25388
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-Beta Arrestin 2 Rabbit mAb [87J31E91] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 2, like arrestin beta 1, was shown to inhibit beta-adrenergic receptor function in vitro. It is expressed at high levels in the central nervous system and may play a role in the regulation of synaptic receptors. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012]

Background

Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 2, like arrestin beta 1, was shown to inhibit beta-adrenergic receptor function in vitro. It is expressed at high levels in the central nervous system and may play a role in the regulation of synaptic receptors. Besides the brain, a cDNA for arrestin beta 2 was isolated from thyroid gland, and thus it may also be involved in hormone-specific desensitization of TSH receptors. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2012]

Cellular Location	Cytoplasm. Nucleus. Cell membrane. Membrane > clathrin-coated pit. Cytoplasmic vesicle. Translocates to the plasma membrane and colocalizes with antagonist-stimulated GPCRs.

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R015220 Anti-Beta Arrestin 2 Rabbit mAb [87J31E91]

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