Immunofluorescence - Anti-Calmodulin 3 Rabbit mAb [68P49S38]

Sample: HeLa cells

The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.5% Triton X-100 for 10 minutes and then blocked with 5% BSA in 0.1% PBS-Tween for 0.5 hours.

Primary antibodies: R015214 at 1:100 dilution and α-tubulin Mouse Monoclonal Antibody (Cat. No. LF209) at 1:100 dilution

Secondary antibodies: Goat anti-Rabbit (488) at 1:1,000 dilution (shown in green) and Goat anti-Mouse (555) at 1:1,000 dilution (shown in red)

Nuclei were stained with DAPI (shown in blue).

Western Blot - Anti-Calmodulin 3 Rabbit mAb [68P49S38]

All lanes: R015214 at 1:8,000 dilution

Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

Lane 2: HepG2 (Human hepatocarcinoma epithelial cell) whole cell lysates

Lane 3: HCT116 (Human colorectal carcinoma epithelial cell) whole cell lysates

Lane 4: 293T (Human embryonic kidney cell) whole cell lysates

Lane 5: K562 (Human chronic myeloid leukemia cell) whole cell lysates

Lane 6: U87 (Human malignant glioblastoma epithelial cells) whole cell lysates

Lane 7: Raw264.7 (Mouse mononuclear macrophage leukemia cell) whole cell lysates

Lane 8: Mouse heart whole tissue lysates

Lane 9: Mouse liver whole tissue lysates

Lane 10: Mouse brain whole tissue lysates

Lane 11: PC-12 (Rat adrenal pheochromocytoma epithelial cell) whole cell lysates

Lane 12: Rat muscle whole tissue lysates

Lane 13: Rat brain whole tissue lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 17 kDa

Observed band size: 17 kDa

Developed using the ECL technique (Cat. No. SQ201).