Western Blot - Anti-PRAS40 Rabbit mAb [56A30P46]
All lanes: R015160 at 1:5,000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2: HepG2 (Human hepatocarcinoma epithelial cell) whole cell lysates
Lane 3: HCT116 (Human colorectal carcinoma epithelial cell) whole cell lysates
Lane 4: SW620 (Human colorectal carcinoma epithelial cell) whole cell lysates
Lane 5: Caco2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates
Lane 6: Jurkat (Human T lymphocytic leukemia cell) whole cell lysates
Lane 7: 293T (Human embryonic kidney cell) whole cell lysates
Lane 8: SCC-9 (Human tongue squamous carcinoma epithelial cell) whole cell lysates
Lane 9: C2C12 (Mouse myoblasts epithelial cell) whole cell lysates
Lane 10: Raw264.7 (Mouse mononuclear macrophage leukemia cell) whole cell lysates
Lane 11: PC-12 (Rat adrenal pheochromocytoma epithelial cell) whole cell lysates
Lysates/proteins at 10 µg per lane.
Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution
Predicted band size: 27 kDa
Observed band size: 40 kDa
Developed using the ECL technique (Cat. No. SQ201).