Anti-Phospho-DNA PKcs (Ser2056) Rabbit mAb [75D61M18]

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Anti-Phospho-DNA PKcs (Ser2056) Rabbit mAb [75D61M18]

Purified Recombinant Rabbit Monoclonal Antibody

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货号	规格	价格
R015021
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Western Blot - Anti-Phospho-DNA PKcs (Ser2056) Rabbit mAb [75D61M18]

All lanes: R015021 at 1:1,000 dilution

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

Lane 2 : HepG2 (Human hepatocarcinoma epithelial cell) whole cell lysates

Lane 3 : HCT116 (Human colorectal carcinoma epithelial cell) whole cell lysates

Lane 4 : SW620 (Human colorectal carcinoma epithelial cell) whole cell lysates

Lane 5 : Caco2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates

Lane 6 : Jurkat (Human T lymphocytic leukemia cell) whole cell lysates

Lane 7 : 293T (Human embryonic kidney cell) whole cell lysates

Lane 8 : SCC-9 (Human tongue squamous carcinoma epithelial cell) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 469 kDa

Observed band size: 469 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunohistochemistry - Anti-Phospho-DNA PKcs (Ser2056) Rabbit mAb [75D61M18]

Sample: Paraformaldehyde-fixed, paraffin embedded human hepatoma tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R015021 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunohistochemistry - Anti-Phospho-DNA PKcs (Ser2056) Rabbit mAb [75D61M18]

Sample: Paraformaldehyde-fixed, paraffin embedded human colonic cancer tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R015021 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

产品信息
说明书
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Product Name	Anti-Phospho-DNA PKcs (Ser2056) Rabbit mAb
Description	Purified Recombinant Rabbit Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P/IF (Tissue-P), ELISA
Dilution	WB 1:1,000~1:2,000; IHC-P 1:100~1:200; IF 1:100~1:200
Reactivity	Human
Host	Rabbit
Clonality	Monoclonal
Clone No.	75D61M18
Isotype	IgG
Label	Unconjugated
Immunogen	A synthesized peptide derived from human Phospho-DNA PKcs (S2056)
Format	Affinity purified monoclonal antibody supplied in PBS with 0.01% sodium azide and 50% glycerol, pH 7.3.

Synonyms	DNA dependent protein kinase catalytic subunit, DNA PK catalytic subunit, DNA-dependent protein kinase catalytic subunit, DNA-PK catalytic subunit, DNA-PKcs, DNAPK, DNAPK catalytic subunit, DNPK 1, DNPK1, Hyper radiosensitivity of murine scid mutation, complementing 1, Hyperradiosensitivity complementing 1, mouse, homolog of 1, HYRC 1, HYRC, HYRC1, IMD26, p350, p460, PKRDC, PRKDC, PRKDC_HUMAN, Protein Kinase DNA Activated Catalytic Polypeptide, XRCC 7, XRCC7.
Molecular weight	Calculated MW: 469 kDa; Observed MW: 469 kDa
Uniprot ID	P78527
Gene ID	5591
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-Phospho-DNA PKcs (Ser2056) Rabbit mAb [75D61M18] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.[provided by RefSeq, Jul 2010]

Cellular Location	Nucleus.

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R015021 Anti-Phospho-DNA PKcs (Ser2056) Rabbit mAb [75D61M18]

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