Western Blot - Anti-CAP2 Rabbit mAb [64T50N48]

All lanes: R014841 at 1:3,000 dilution

Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

Lane 2: HepG2 (Human hepatocarcinoma epithelial cell) whole cell lysates

Lane 3: HCT116 (Human colorectal carcinoma epithelial cell) whole cell lysates

Lane 4: U2OS (Human osteosarcoma epithelial cell) whole cell lysates

Lane 5: Caco2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates

Lane 6: 293T (Human embryonic kidney cell) whole cell lysates

Lane 7: SCC-9 (Human tongue squamous carcinoma epithelial cell) whole cell lysates

Lane 8: Raw264.7 (Mouse mononuclear macrophage leukemia cell) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 53 kDa

Observed band size: 53 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunohistochemistry - Anti-CAP2 Rabbit mAb [64T50N48]

Sample: Paraformaldehyde-fixed, paraffin embedded rat heart tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R014841 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunohistochemistry - Anti-CAP2 Rabbit mAb [64T50N48]

Sample: Paraformaldehyde-fixed, paraffin embedded human endometrial carcinoma tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R014841 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunohistochemistry - Anti-CAP2 Rabbit mAb [64T50N48]

Sample: Paraformaldehyde-fixed, paraffin embedded mouse muscle tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R014841 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.