Anti-Phospho-Smad1/5/9 (Ser463/Ser465/Ser467) Rabbit mAb [64M70A53]

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Anti-Phospho-Smad1/5/9 (Ser463/Ser465/Ser467) Rabbit mAb [64M70A53]

Purified Recombinant Rabbit Monoclonal Antibody

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货号	规格	价格
R014754
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Western Blot - Anti-Phospho-Smad1/5/9 (Ser463/Ser465/Ser467) Rabbit mAb [64M70A53]

All lanes: R014754 at 1:1,000 dilution

Lane 1: HepG2 (Human hepatocarcinoma epithelial cell) whole cell lysates

Lane 2: HCT116 (Human colorectal carcinoma epithelial cell) whole cell lysates

Lane 3: A431 (Human epidermoid teratoma cell line) whole cell lysates

Lane 4: Raw264.7 (Mouse mononuclear macrophage leukemia cell) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunofluorescence - Anti-Phospho-Smad1/5/9 (Ser463/Ser465/Ser467) Rabbit mAb [64M70A53]

Sample: Hela cells

The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.5% Triton X-100 for 10 minutes and then blocked with 5% BSA in 0.1% PBS-Tween for 0.5 hours.

Primary antibodies: R014754 at 1:100 dilution and α-tubulin Mouse Monoclonal Antibody (Cat. No. LF209) at 1:100 dilution

Secondary antibodies: Goat anti-Rabbit (488) at 1:1,000 dilution (shown in green) and Goat anti-Mouse (555) at 1:1,000 dilution (shown in red)

Nuclei were stained with DAPI (shown in blue).

Immunohistochemistry - Anti-Phospho-Smad1/5/9 (Ser463/Ser465/Ser467) Rabbit mAb [64M70A53]

Sample: Paraformaldehyde-fixed, paraffin embedded rat bladder tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R014754 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunohistochemistry - Anti-Phospho-Smad1/5/9 (Ser463/Ser465/Ser467) Rabbit mAb [64M70A53]

Sample: Paraformaldehyde-fixed, paraffin embedded human endometrial carcinoma tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R014754 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

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Product Name	Anti-Phospho-Smad1/5/9 (Ser463/Ser465/Ser467) Rabbit mAb
Description	Purified Recombinant Rabbit Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P/IF (Tissue-P), IF (Cell)/ICC, ELISA
Dilution	WB 1:1,000~1:2,000; IHC-P 1:100~1:200; IF 1:100~1:200
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Monoclonal
Clone No.	64M70A53
Isotype	IgG
Label	Unconjugated
Immunogen	A synthesized peptide derived from human Smad5
Format	Affinity purified monoclonal antibody supplied in PBS with 0.01% sodium azide and 50% glycerol, pH 7.3.

Synonyms	AI528653, BSP1, DKFZp781C1895, DKFZp781O1323, Dwarfin A, Dwf A, Dwfc, hSMAD1, hSmad5, JV4 1, JV41, JV5 1, MAD (mothers against decapentaplegic, Drosophila) homolog 1, MAD (mothers against decapentaplegic, Drosophila) homolog 9, MAD homolog 1, MAD homolog 9, MAD homolog1 (mothers against decapentaplegic, Drosophila), Mad related protein 1, MAD, mothers against decapentaplegic homolog 9, Madh1, MADH5, MADH6, MADH9, MADR1, Mlp1, Mothers against decapentaplegic, drosophila, homolog of, 9, Mothers against DPP homolog 1, Mothers against DPP homolog 9, MusMLP, OTTHUMP00000178565, OTTMUSP00000022970, OTTMUSP00000022971, OTTMUSP00000022972, OTTMUSP0000002324, OTTMUSP00000023428, Sma- and Mad-related protein 1, SMAD, SMAD family member 5, SMAD8, SMAD8A, SMAD8B, TGF beta signaling protein 1, Transforming growth factor-beta signaling protein 1.
Molecular weight	Calculated MW: 52 kDa; Observed MW: 52 kDa
Uniprot ID	Q99717
Gene ID	4090
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-Phospho-Smad1/5/9 (Ser463/Ser465/Ser467) Rabbit mAb [64M70A53] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).

Cellular Location	Smad1: Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane. SMAD5: Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. SMAD9: Cytoplasm. Nucleus. In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with SMAD4.

Cellular Location

Smad1: Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. Co-localizes with LEMD3 at the nucleus inner membrane. SMAD5: Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. SMAD9: Cytoplasm. Nucleus. In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with SMAD4.

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R014754 Anti-Phospho-Smad1/5/9 (Ser463/Ser465/Ser467) Rabbit mAb [64M70A53]

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