Western Blot - Anti-Pyrophosphatase 1 Rabbit mAb [65Q17B81]

All lanes: R014742 at 1:1,000 dilution

Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

Lane 2: HepG2 (Human hepatocarcinoma epithelial cell) whole cell lysates

Lane 3: HCT116 (Human colorectal carcinoma epithelial cell) whole cell lysates

Lane 4: T24 (Human bladder cancer epithelial cell) whole cell lysates

Lane 5: Jurkat (Human T lymphocytic leukemia cell) whole cell lysates

Lane 6: 293T (Human embryonic kidney cell) whole cell lysates

Lane 7: Raw264.7 (Mouse mononuclear macrophage leukemia cell) whole cell lysates

Lane 8: PC-12 (Rat adrenal pheochromocytoma epithelial cell) whole cell lysates

Lane 9: Rat brain whole tissue lysates

Lane10: Rat heart whole tissue lysates

Lane11: Rat kidney whole tissue lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 33 kDa

Observed band size: 33 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunohistochemistry - Anti-Pyrophosphatase 1 Rabbit mAb [65Q17B81]

Sample: Paraformaldehyde-fixed, paraffin embedded rat lung tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R014742 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunohistochemistry - Anti-Pyrophosphatase 1 Rabbit mAb [65Q17B81]

Sample: Paraformaldehyde-fixed, paraffin embedded human colonic cancer tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R014742 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunohistochemistry - Anti-Pyrophosphatase 1 Rabbit mAb [65Q17B81]

Sample: Paraformaldehyde-fixed, paraffin embedded mouse uterus tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R014742 at 1:100 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.