Anti-Rho A/B/C Rabbit mAb [17E16R90]

抗体系列

Anti-Rho A/B/C Rabbit mAb [17E16R90]

Purified Recombinant Rabbit Monoclonal Antibody

一键复制产品信息

货号	规格	价格
R014568
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

立即购买

Western Blot - Anti-Rho A/B/C Rabbit mAb [17E16R90]

All lanes: R014568 at 1:1,000 dilution

Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

Lane 2: Raw264.7 (Mouse mononuclear macrophage leukemia cell) whole cell lysates

Lane 3: C2C12 (Mouse myoblasts epithelial cell) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 22 kDa

Observed band size: 20 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunofluorescence - Anti-Rho A/B/C Rabbit mAb [17E16R90]

Sample: A431 cells

The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.5% Triton X-100 for 10 minutes and then blocked with 5% BSA in 0.1% PBS-Tween for 0.5 hours.

Primary antibodies: R014568 at 1:100 dilution and α-tubulin Mouse Monoclonal Antibody (Cat. No. LF209) at 1:100 dilution

Secondary antibodies: Goat anti-Rabbit (488) at 1:1,000 dilution (shown in green) and Goat anti-Mouse (555) at 1:1,000 dilution (shown in red)

Nuclei were stained with DAPI (shown in blue).

Product Name	Anti-Rho A/B/C Rabbit mAb
Description	Purified Recombinant Rabbit Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IF (Cell)/ICC, ELISA
Dilution	WB 1:1,000~1:2,000; IF 1:100~1:200
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal
Clone No.	17E16R90
Isotype	IgG
Label	Unconjugated
Immunogen	Recombinant protein of human Rho C
Format	Affinity purified monoclonal antibody supplied in PBS with 0.01% sodium azide and 50% glycerol, pH 7.3.

Synonyms	AA017882, AI324259,, Aplysia ras related homolog 9 (RhoC), Aplysia ras-related homolog 12, Aplysia RAS-related homolog 6, Aplysia RAS-related homolog, ARH12, ARH6, ARH9, ARHA, ARHB, ARHC, H12, H6, H9, MGC117867, MGC1448, MGC61427, MST081, MSTP081, Oncogene RHO H12, Oncogene RHO H6, Oncogene RHO H9, OTTHUMP00000013675, OTTHUMP00000013676, OTTHUMP00000013802, OTTHUMP00000013805, OTTHUMP00000013807, OTTHUMP00000013809, OTTMUSP00000025086, OTTMUSP00000025087, Ras homolog 9 (RhoC), Ras homolog gene family, member A, Ras homolog gene family, member B, Ras homolog gene family, member C, RAS-related homolog 9, Rh -related GTP binding protein RhoC, Rho cDNA clone 12, rho cDNA clone 6, rho cDNA clone 9, Rho related GTP binding protein RhoB, Rho related GTP binding protein RhoC, RHO12, RHOA, RHOB, RHOC, rhoC GTPase, RHOH12, RHOH6, RHOH9, RP11-426L16.4, small GTP binding protein RhoA, Small GTP binding protein RhoC, Transforming protein RhoA.
Molecular weight	Calculated MW: 22 kDa; Observed MW: 20 kDa
Uniprot ID	P08134
Gene ID	389
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-Rho A/B/C Rabbit mAb [17E16R90] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Background

This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Cellular Location	Rho A: Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm; cytoskeleton. Rho B: Late endosome membrane; Lipid-anchor. Cell membrane; Lipid-anchor. Nucleus. Rho C: Cell membrane; Lipid-anchor; Cytoplasmic side.

说明书下载

R014568 Anti-Rho A/B/C Rabbit mAb [17E16R90]

总篇数：加载中...

总影响因子：加载中...

最高影响因子：加载中...

加载中...

没有更多数据

产品中心

联系雅酶

上海市闵行区新骏环路760号1号楼3层

info@epizyme.cn

400-058-8030

技术支持(电话/微信)：

张老师 18217238522

罗老师 15021027255

李老师 18301721288

如有任何关于雅酶产品问题
欢迎来信咨询

雅酶微信公众号

抗体

蛋白提取与定量

蛋白电泳

蛋白免疫印迹

蛋白表达纯化与分离

蛋白质

ELISA试剂盒

核酸相关实验

微生物培养

设备与耗材

细胞培养

网站首页