Western Blot - Anti-RhoGDI Rabbit mAb [70T67P99]

All lanes: R014531 at 1:1,000 dilution

Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

Lane 2: HepG2 (Human hepatocarcinoma epithelial cell) whole cell lysates

Lane 3: HCT116 (Human colorectal carcinoma epithelial cell)   whole cell lysates

Lane 4: A431 (Human epidermoid teratoma cell line) whole cell lysates

Lane 5: Rat spleen whole tissue lysates

Lane 6: Rat heart whole tissue lysates

Lane 7: Rat muscle whole tissue lysates

Lane 8: Mouse brain whole tissue lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 23 kDa

Observed band size: 26 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunohistochemistry - Anti-RhoGDI Rabbit mAb [70T67P99]

Sample: Paraformaldehyde-fixed, paraffin embedded human breast cancer tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R014531 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.