Anti-GABARAP Rabbit mAb [87G22C38]

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Anti-GABARAP Rabbit mAb [87G22C38]

Purified Recombinant Rabbit Monoclonal Antibody

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货号	规格	价格
R014521
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Western Blot - Anti-GABARAP Rabbit mAb [87G22C38]

All lanes: R014521 at 1:1,000 dilution

Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

Lane 2: HepG2 (Human hepatocarcinoma epithelial cell) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG(H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 14 kDa

Observed band size: 17 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunofluorescence - Anti-GABARAP Rabbit mAb [87G22C38]

Sample: Hela cells

The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.5% Triton X-100 for 10 minutes and then blocked with 5% BSA in 0.1% PBS-Tween for 0.5 hours.

Primary antibodies: R014521 at 1:100 dilution and α-tubulin Mouse Monoclonal Antibody (Cat. No. LF209) at 1:100 dilution

Secondary antibodies: Goat anti-Rabbit (488) at 1:1,000 dilution (shown in green) and Goat anti-Mouse (555) at 1:1,000 dilution (shown in red)

Nuclei were stained with DAPI (shown in blue).

Western Blot - Anti-GABARAP Rabbit mAb [87G22C38]

All lanes: R014521 at 1:1,000 dilution

Lane 1: C2C12 (Mouse myoblasts epithelial cell) whole cell lysates

Lane 2: Raw264.7 (Mouse mononuclear macrophage leukemia cell)whole cell lysates

Lane 3: PC-12(Rat adrenal pheochromocytoma epithelial cell)whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG(H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 14 kDa

Observed band size: 17 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunohistochemistry - Anti-GABARAP Rabbit mAb [87G22C38]

Sample: Paraformaldehyde-fixed, paraffin embedded human hepatoma tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R014521 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

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Product Name	Anti-GABARAP Rabbit mAb
Description	Purified Recombinant Rabbit Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	ELISA, WB, IHC-P/IF (Tissue-P), IF (Cell)/ICC
Dilution	WB 1:1,000~1:2,000; IHC-P 1:100~1:200; IF 1:100~1:200
Reactivity	Human, Rat, Mouse
Host	Rabbit
Clonality	Monoclonal
Clone No.	87G22C38
Isotype	IgG
Label	Unconjugated
Immunogen	A synthesized peptide derived from human GABARAP
Format	Affinity purified monoclonal antibody supplied in PBS with 0.01% sodium azide and 50% glycerol, pH 7.3.

Synonyms	ATG8A, FLC 3B, FLC3B, FLJ25768, GABA type A receptor associated protein, GABA(A) receptor associated protein, GABA(A) receptor-associated protein, GABARAP a, GABARAP, Gamma aminobutyric acid receptor associated protein, Gamma-aminobutyric acid receptor-associated protein, GBRAP_HUMAN, MGC120154, MGC120155, MM 46, MM46.
Molecular weight	Calculated MW: 14 kDa; Observed MW: 17 kDa
Uniprot ID	O95166
Gene ID	11337
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-GABARAP Rabbit mAb [87G22C38] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	GABARAP is cleaved at its carboxyl terminus, which leads to conjugation by either of the phospholipids phosphatidylethanolamine or phosphatidylserine. This processing converts GABARAP from a type I to a type II membrane bound form involved in autophagosome biogenesis. Processing of GABARAP involves cleavage by Atg4 family members followed by conjugation by the E1 and E2 like enzymes Atg7 and Atg3.

Background

GABARAP is cleaved at its carboxyl terminus, which leads to conjugation by either of the phospholipids phosphatidylethanolamine or phosphatidylserine. This processing converts GABARAP from a type I to a type II membrane bound form involved in autophagosome biogenesis. Processing of GABARAP involves cleavage by Atg4 family members followed by conjugation by the E1 and E2 like enzymes Atg7 and Atg3.

Cellular Location	Endomembrane system. Cytoplasm, cytoskeleton. Golgi apparatus membrane. Cytoplasmic vesicle, autophagosome. Largely associated with intracellular membrane structures including the Golgi apparatus and postsynaptic cisternae. Colocalizes with microtubules (By similarity). Localizes also to discrete punctae along the ciliary axoneme (By similarity).

Tissue Location	Heart, brain, placenta, liver, skeletal muscle, kidney and pancreas.

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R014521 Anti-GABARAP Rabbit mAb [87G22C38]

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