Western Blot - Anti-CYP27B1 Rabbit mAb [22D32P28]

All lanes: R014237 at 1:1,000 dilution

Lane 1: HepG2 (Human hepatocarcinoma epithelial cell) whole cell lysates

Lane 2: HCT116 (Human colorectal carcinoma epithelial cell) whole cell lysates

Lane 3: A431 (Human epidermoid teratoma cell line) whole cell lysates

Lane 4: Rat kidney whole tissue lysates

Lane 5: Mouse brain whole tissue lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG(H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 57 kDa

Observed band size: 57 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunohistochemistry - Anti-CYP27B1 Rabbit mAb [22D32P28]

Sample: Paraformaldehyde-fixed, paraffin embedded human renal carcinoma tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R014237 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunohistochemistry - Anti-CYP27B1 Rabbit mAb [22D32P28]

Sample: Paraformaldehyde-fixed, paraffin embedded mouse kidney tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R014237 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.