Western Blot - Anti-Phospho-SIRT1 (Thr530) Rabbit mAb [58C48O07]

All lanes: R014158 at 1:1,000 dilution

Lane 1: A431 (Human epidermoid teratoma cell line) whole cell lysates

Lane 2: T24 (Human bladder cancer epithelial cell) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG(H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 82 kDa

Observed band size: 120 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunohistochemistry - Anti-Phospho-SIRT1 (Thr530) Rabbit mAb [58C48O07]

Sample: Paraformaldehyde-fixed, paraffin embedded human endometrial carcinoma tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R014158 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunohistochemistry - Anti-Phospho-SIRT1 (Thr530) Rabbit mAb [58C48O07]

Sample: Paraformaldehyde-fixed, paraffin embedded mouse muscle tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R014158 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.