Anti-Phospho-MEK1 (Thr292) Rabbit mAb [68A07N13]

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Anti-Phospho-MEK1 (Thr292) Rabbit mAb [68A07N13]

Purified Recombinant Rabbit Monoclonal Antibody

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货号	规格	价格
R013985
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Western Blot - Anti-Phospho-MEK1 (Thr292) Rabbit mAb [68A07N13]

All lanes: R013985 at 1:1,000 dilution

Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG(H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 43 kDa

Observed band size: 45 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunohistochemistry - Anti-Phospho-MEK1 (Thr292) Rabbit mAb [68A07N13]

Sample: Paraformaldehyde-fixed, paraffin embedded human colonic cancer tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R013985 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunofluorescence - Anti-Phospho-MEK1 (Thr292) Rabbit mAb [68A07N13]

Sample: HeLa cells

The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.5% Triton X-100 for 10 minutes and then blocked with 5% BSA in 0.1% PBS-Tween for 0.5 hours.

Primary antibodies: R013985 at 1:100 dilution and α-tubulin Mouse Monoclonal Antibody (Cat. No. LF209) at 1:100 dilution

Secondary antibodies: Goat anti-Rabbit (488) at 1:1,000 dilution (shown in green) and Goat anti-Mouse (555) at 1:1,000 dilution (shown in red)

Nuclei were stained with DAPI (shown in blue).

Western Blot -Anti-Phospho-MEK1 (Thr292) Rabbit mAb [68A07N13]

All lanes: R013985 at 1:1,000 dilution

Lane 1: C2C12 (Mouse myoblasts epithelial cell) whole cell lysates

Lane 2: Raw264.7 (Mouse mononuclear macrophage leukemia cell)whole cell lysates

Lane 3: PC-12(Rat adrenal pheochromocytoma epithelial cell)whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG(H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 43 kDa

Observed band size: 45 kDa

Developed using the ECL technique (Cat. No. SQ201).

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Product Name	Anti-Phospho-MEK1 (Thr292) Rabbit mAb
Description	Purified Recombinant Rabbit Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	ELISA, WB, IHC-P/IF (Tissue-P), IF (Cell)/ICC
Dilution	WB 1:1,000~1:2,000; IHC-P 1:100~1:200; IF 1:100~1:200
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal
Clone No.	68A07N13
Isotype	IgG
Label	Unconjugated
Immunogen	A synthesized peptide derived from human MEK1
Format	Affinity purified monoclonal antibody supplied in PBS with 0.01% sodium azide and 50% glycerol, pH 7.3.

Synonyms	Dual specificity mitogen activated protein kinase kinase 1, Dual specificity mitogen-activated protein kinase kinase 1, ERK activator kinase 1, MAP kinase kinase 1, MAP kinase/Erk kinase 1, MAP2K1, MAPK/ERK kinase 1, MAPKK 1, MAPKK1, MEK 1, Mek1, MEKK1, Mitogen activated protein kinase kinase 1, MKK 1, MKK1, MP2K1_HUMAN, PRKMK1, Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1), Protein kinase mitogen activated, kinase 1, protein kinase mitogen-activated kinase 1.
Molecular weight	Calculated MW: 43 kDa; Observed MW: 45 kDa
Uniprot ID	Q02750
Gene ID	5604
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-Phospho-MEK1 (Thr292) Rabbit mAb [68A07N13] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation. Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.

Background

MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation. Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.

Tissue Location	Widely expressed, with extremely low levels in brain.

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R013985 Anti-Phospho-MEK1 (Thr292) Rabbit mAb [68A07N13]

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