Western Blot - Anti-Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Rabbit mAb [67S45G56]

All lanes: R013960 at 1:4,000 dilution

Lane 1: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG(H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 53 kDa

Observed band size: 40 kDa, 42 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunohistochemistry - Anti-Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Rabbit mAb [67S45G56]

Sample: Paraformaldehyde-fixed, paraffin embedded human gastric cancer tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R013960 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunohistochemistry - Anti-Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Rabbit mAb [67S45G56]

Sample: Paraformaldehyde-fixed, paraffin embedded rat bladder tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R013960 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.