Anti-NF-KB p105/p50 Rabbit mAb [72Q88S90]

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Anti-NF-KB p105/p50 Rabbit mAb [72Q88S90]

Purified Recombinant Rabbit Monoclonal Antibody

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货号	规格	价格
R013948
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Western Blot - Anti-NF-KB p105/p50 Rabbit mAb [72Q88S90]

All lanes: R013948 at 1:1,000 dilution

Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

Lane 2: HepG2 (Human hepatocarcinoma epithelial cell) whole cell lysates

Lane 3: HCT116 (Human colorectal carcinoma epithelial cell) whole cell lysates

Lane 4: A431 (Human epidermoid teratoma cell line) whole cell lysates

Lane 5: T24 (Human bladder cancer epithelial cell) whole cell lysates

Lane 6: Rat heart whole tissue lysates

Lane 7: Mouse muscle whole tissue lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG(H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 50 kDa

Observed band size: 50 kDa, 120 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunofluorescence - Anti-NF-KB p105/p50 Rabbit mAb [72Q88S90]

Sample: Hela cells

The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.5% Triton X-100 for 10 minutes and then blocked with 5% BSA in 0.1% PBS-Tween for 0.5 hours.

Primary antibodies: R013948 at 1:100 dilution and α-tubulin Mouse Monoclonal Antibody (Cat. No. LF209) at 1:100 dilution

Secondary antibodies: Goat anti-Rabbit (488) at 1:1,000 dilution (shown in green) and Goat anti-Mouse (555) at 1:1,000 dilution (shown in red)

Nuclei were stained with DAPI (shown in blue).

Immunohistochemistry - Anti-NF-KB p105/p50 Rabbit mAb [72Q88S90]

Sample: Paraformaldehyde-fixed, paraffin embedded human colorectal carcinoma tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R0139487 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunohistochemistry - Anti-NF-KB p105/p50 Rabbit mAb [72Q88S90]

Sample: Paraformaldehyde-fixed, paraffin embedded mouse lung tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R0139487 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

产品信息
说明书
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Product Name	Anti-NF-KB p105/p50 Rabbit mAb
Description	Purified Recombinant Rabbit Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	ELISA, WB, IHC-P/IF (Tissue-P), IF (Cell)/ICC
Dilution	WB 1:1,000~1:2,000; IHC-P 1:200; IF 1:100
Reactivity	Mouse, Human, Rat
Host	Rabbit
Clonality	Monoclonal
Clone No.	72Q88S90
Isotype	IgG
Label	Unconjugated
Immunogen	A synthesized peptide derived from human NF-κB (p105/p50)
Format	Affinity purified monoclonal antibody supplied in PBS with 0.01% sodium azide and 50% glycerol, pH 7.3.

Synonyms	NF-κB1, NF-kappa-B1, p84/NF-kappa-B1 p98, NFKB1, DNA-binding factor KBF1, EBP-1, NFkB-p50, NFKB1_HUMAN, Nuclear factor NF-kappa-B p105 subunit, DNA-binding factor KBF1, Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1, Nuclear factor NF-kappa-B p50 subunit, NFkB p105/p50, nuclear factor kappa B subunit 1, NF-kB, CVID12, NF-kB1, NFKB-p50, NFkappaB, NF-kappaB, NFKB-p105, NF-kappa beta, DKFZp686C01211, MGC54151.
Molecular weight	Calculated MW: 50 kDa; Observed MW: 50 kDa, 120 kDa
Uniprot ID	P19838
Gene ID	4790
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-NF-KB p105/p50 Rabbit mAb [72Q88S90] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	NFkB-p105 a transcription factor of the nuclear factor-kappaB ( NFkB) group. Undergoes cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of NFkB. NFkB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products.

Background

NFkB-p105 a transcription factor of the nuclear factor-kappaB ( NFkB) group. Undergoes cotranslational processing by the 26S proteasome to produce a 50 kD protein. The 105 kD protein is a Rel protein-specific transcription inhibitor and the 50 kD protein is a DNA binding subunit of NFkB. NFkB is a transcription regulator that is activated by various intra- and extra-cellular stimuli such as cytokines, oxidant-free radicals, ultraviolet irradiation, and bacterial or viral products.

Cellular Location	Nucleus. Cytoplasm. Note=Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B).

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R013948 Anti-NF-KB p105/p50 Rabbit mAb [72Q88S90]

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