Anti-IKK beta Rabbit mAb [64H71B09]

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Anti-IKK beta Rabbit mAb [64H71B09]

Purified Recombinant Rabbit Monoclonal Antibody

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货号	规格	价格
R013865
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Western Blot - Anti-IKK beta Rabbit mAb [64H71B09]

All lanes: R013865 at 1:1,000 dilution

Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

Lane 2: Jurkat (Human T lymphocytic leukemia cell) whole cell lysates

Lane 3: HCT116 (Human colorectal carcinoma epithelial cell) whole cell lysates

Lane 4: SCC-9 (Human tongue squamous carcinoma epithelial cell) whole cell lysates

Lane 5: U2OS (Human osteosarcoma epithelial cell) whole cell lysates

Lane 6: SW620 (Human colorectal carcinoma epithelial cell) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG(H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 87 kDa

Observed band size: 87 kDa

Developed using the ECL technique (Cat. No. SQ201).

Product Name	Anti-IKK beta Rabbit mAb
Description	Purified Recombinant Rabbit Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, ELISA
Dilution	WB 1:1,000~1:2,000
Reactivity	Human
Host	Rabbit
Clonality	Monoclonal
Clone No.	64H71B09
Isotype	IgG
Label	Unconjugated
Immunogen	A synthesized peptide derived from human IKK beta
Format	Affinity purified monoclonal antibody supplied in PBS with 0.01% sodium azide and 50% glycerol, pH 7.3.

Synonyms	IKBKB, IKKB, Inhibitor of nuclear factor kappa-B kinase subunit beta, I-kappa-B-kinase beta, IKK-B, IKK-beta, IkBKB, I-kappa-B kinase 2, IKK2, Nuclear factor NF-kappa-B inhibitor kinase beta, NFKBIKB.
Molecular weight	Calculated MW: 87 kDa; Observed MW: 87 kDa
Uniprot ID	O14920
Gene ID	3551
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-IKK beta Rabbit mAb [64H71B09] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits.

Background

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits.

Cellular Location	Cytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts.

Tissue Location	Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.

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R013865 Anti-IKK beta Rabbit mAb [64H71B09]

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