Western Blot - Anti-PCNA Rabbit mAb [75B19K12]

All lanes: R013861 at 1:1,000 dilution

Lane 1: HCT116 (Human colorectal carcinoma epithelial cell) whole cell lysates

Lane 2: SCC-9 (Human tongue squamous carcinoma epithelial cell) whole cell lysates

Lane 3: U2OS (Human osteosarcoma epithelial cell) whole cell lysates

Lane 4: SW620 (Human colorectal carcinoma epithelial cell) whole cell lysates

Lane 5: 293T (Human embryonic kidney cell) whole cell lysates

Lane 6: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates

Lane 7: Rat heart whole tissue lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG(H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunohistochemistry - Anti-PCNA Rabbit mAb [75B19K12]

Sample: Paraformaldehyde-fixed, paraffin embedded human colorectal carcinoma tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R013861 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunohistochemistry - Anti-PCNA Rabbit mAb [75B19K12]

Sample: Paraformaldehyde-fixed, paraffin embedded rat testis tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R013861 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.