Western Blot - Anti-Myosin light chain 3 Rabbit mAb [48M01D83]

All lanes: R013829 at 1:2,000 dilution

Lane 1: Rat heart whole tissue lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG(H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 22 kDa

Observed band size: 22 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunofluorescence - Anti-Myosin light chain 3 Rabbit mAb [48M01D83]

Sample: Paraformaldehyde-fixed, paraffin embedded mouse thigh muscle tissue

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R013829 at 1:100 dilution

Secondary antibody: Goat anti-Rabbit (488) at 1:1,000 dilution (shown in green)

Nuclei were stained with DAPI (shown in blue).

Immunohistochemistry - Anti-Myosin light chain 3 Rabbit mAb [48M01D83]

Sample: Paraformaldehyde-fixed, paraffin embedded mouse heart tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R013829 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.