Anti-HDAC4 Rabbit mAb [53C01M23]

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Anti-HDAC4 Rabbit mAb [53C01M23]

Purified Recombinant Rabbit Monoclonal Antibody

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货号	规格	价格
R013809
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Western Blot - Anti-HDAC4 Rabbit mAb [53C01M23]

All lanes: R013809 at 1:1,000 dilution

Lane 1: Jurkat (human T lymphocytic leukemia cell) whole cell lysates

Lane 2: HCT116 (human colorectal carcinoma epithelial cell) whole cell lysates

Lane 3: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysates

Lane 4: Rat brain whole tissue lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG(H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 119 kDa

Observed band size: 140 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunofluorescence - Anti-HDAC4 Rabbit mAb [53C01M23]

Sample: Hela cells

The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.5% Triton X-100 for 10 minutes and then blocked with 5% BSA in 0.1% PBS-Tween for 0.5 hours.

Primary antibodies: R013809 at 1:50 dilution and α-tubulin Mouse Monoclonal Antibody (Cat. No. LF209) at 1:50 dilution

Secondary antibodies: Goat anti-Rabbit (488) at 1:1,000 dilution (shown in green) and Goat anti-Mouse (555) at 1:1,000 dilution (shown in red)

Nuclei were stained with DAPI (shown in blue).

Immunohistochemistry - Anti-HDAC4 Rabbit mAb [53C01M23]

Sample: Paraformaldehyde-fixed, paraffin embedded rat testis tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R013809 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunohistochemistry - Anti-HDAC4 Rabbit mAb [53C01M23]

Sample: Paraformaldehyde-fixed, paraffin embedded mouse kidney tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R013809 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Western Blot - Anti-HDAC4 Rabbit mAb [53C01M23]

All lanes: R013809 at 1:1,000 dilution

Lane 1: C2C12 (Mouse myoblasts epithelial cell) whole cell lysates

Lane 2: Raw264.7 (Mouse mononuclear macrophage leukemia cell)whole cell lysates

Lane 3: PC-12(Rat adrenal pheochromocytoma epithelial cell)whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG(H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 119 kDa

Observed band size: 140 kDa

Developed using the ECL technique (Cat. No. SQ201).

产品信息
说明书
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Product Name	Anti-HDAC4 Rabbit mAb
Description	Purified Recombinant Rabbit Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	IF (Cell)/ICC, ELISA, WB, IHC-P/IF (Tissue-P)
Dilution	WB 1:1,000~1:2,000; IHC-P 1:100~1:200; IF 1:100~1:200
Reactivity	Mouse, Rat, Human
Host	Rabbit
Clonality	Monoclonal
Clone No.	53C01M23
Isotype	IgG
Label	Unconjugated
Immunogen	Recombinant protein of human HDAC4
Format	Affinity purified monoclonal antibody supplied in PBS with 0.01% sodium azide and 50% glycerol, pH 7.3.

Synonyms	AHO3, BDMR, EC 3.5.1.98, HA6116, HD 4, HD4, HDAC 4, HDAC A, HDAC4, HDAC4_HUMAN, HDACA, Histone deacetylase 4, Histone Deacetylase A, KIAA0288.
Molecular weight	Calculated MW: 119 kDa; Observed MW: 140 kDa
Uniprot ID	P56524 , Q6NZM9
Gene ID	9759 , 208727
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-HDAC4 Rabbit mAb [53C01M23] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3. [provided by RefSeq, Jul 2008]

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3. [provided by RefSeq, Jul 2008]

Cellular Location	Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4. The nuclear localization probably depends on sumoylation.

Cellular Location

Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. Upon muscle cells differentiation, it accumulates in the nuclei of myotubes, suggesting a positive role of nuclear HDAC4 in muscle differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-246, Ser-467 and Ser-632 by CaMK4. The nuclear localization probably depends on sumoylation.

Tissue Location	Ubiquitous.

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R013809 Anti-HDAC4 Rabbit mAb [53C01M23]

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