Western Blot - Anti-PSD95 Rabbit mAb [21E45F92]

All lanes: R013801 at 1:1,000 dilution

Lane 1: Balb/c mouse brain whole tissue lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG(H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 80 kDa

Observed band size: 75 kDa, 100 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunofluorescence - Anti-PSD95 Rabbit mAb [21E45F92]

Sample: Paraformaldehyde-fixed, paraffin embedded mouse brain tissue (Sections of cerebral cortex and corpus callosum)

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R013801 at 1:100 dilution

Secondary antibody: Goat anti-Rabbit (488) at 1:1,000 dilution (shown in green)

Nuclei were stained with DAPI (shown in blue).

Figurelegends:

a: Whole scene of PSD95 stained mouse cerebral cortex and corpus callosum.

b: 40× view. Green: PSD95; Blue: DAPI.

c: Mouse brain staining with secondary antibody only for control experiment.

d. Enlarge view of b.

e: DAPI of b.

Immunofluorescence - Anti-PSD95 Rabbit mAb [21E45F92]

Sample: SY5Y cells

The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.5% Triton X-100 for 10 minutes and then blocked with 5% BSA in 0.1% PBS-Tween for 0.5 hours.

Primary antibodies: R013801 at 1:100 dilution and α-tubulin Mouse Monoclonal Antibody (Cat. No. LF209) at 1:100 dilution

Secondary antibodies: Goat anti-Rabbit (488) at 1:1,000 dilution (shown in green) and Goat anti-Mouse (555) at 1:1,000 dilution (shown in red)

Nuclei were stained with DAPI (shown in blue).