Anti-p38 Rabbit mAb [49C01B92]

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Anti-p38 Rabbit mAb [49C01B92]

Purified Recombinant Rabbit Monoclonal Antibody

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货号	规格	价格
R013794
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Western Blot - Anti-p38 Rabbit mAb [49C01B92]

All lanes: R013794 at 1:1,000 dilution

Lane 1: Jurkat (human T lymphocytic leukemia cell) whole cell lysatess

Lane 2: HCT116 (human colorectal carcinoma epithelial cell) whole cell lysatess

Lane 3: T24 (human bladder cancer epithelial cell) whole cell lysatess

Lane 4: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysates

Lane 5: RAW264.7 (mouse mononuclear macrophage leukemia epithelial cell)

Lane 6: SW620 (human colorectal carcinoma epithelial cell) whole cell lysates

Lane 7: Rat kidney whole tissue lysates

Lane 8: Rat spleen whole tissue lysates

Lane 9: Balb/c mouse brain whole tissue lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG(H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 41 kDa

Observed band size: 41 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunohistochemistry - Anti-p38 Rabbit mAb [49C01B92]

Sample: Paraformaldehyde-fixed, paraffin embedded rat heart tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R013794 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunohistochemistry - Anti-p38 Rabbit mAb [49C01B92]

Sample: Paraformaldehyde-fixed, paraffin embedded human renal cancer tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R013794 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunofluorescence - Anti-p38 Rabbit mAb [49C01B92]

Sample: Hela cells

The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.5% Triton X-100 for 10 minutes and then blocked with 5% BSA in 0.1% PBS-Tween for 0.5 hours.

Primary antibodies: R013794 at 1:100 dilution and α-tubulin Mouse Monoclonal Antibody (Cat. No. LF209) at 1:100 dilution

Secondary antibodies: Goat anti-Rabbit (488) at 1:1,000 dilution (shown in green) and Goat anti-Mouse (555) at 1:1,000 dilution (shown in red)

Nuclei were stained with DAPI (shown in blue).

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Product Name	Anti-p38 Rabbit mAb
Description	Purified Recombinant Rabbit Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	ELISA, WB, IHC-P/IF (Tissue-P), IF (Cell)/ICC
Dilution	WB 1:1,000~1:2,000; IHC-P 1:200; IF 1:100
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Monoclonal
Clone No.	49C01B92
Isotype	IgG
Label	Unconjugated
Immunogen	A synthesized peptide derived from human p38
Format	Affinity purified monoclonal antibody supplied in PBS with 0.01% sodium azide and 50% glycerol, pH 7.3.

Synonyms	RK, CSBP, EXIP, Mxi2, CSBP1, CSBP2, CSPB1, PRKM14, PRKM15, SAPK2A, CSAID Binding Protein 1, CSAID binding protein, CSAID-binding protein, Cytokine suppressive anti-inflammatory drug-binding protein, MAP kinase 14, MAP kinase MXI2, MAP kinase p38 alpha, MAPK 14, MAX-interacting protein 2, Mitogen activated protein kinase p38 alpha, Mitogen-activated protein kinase 14, Mitogen-activated protein kinase p38 alpha, p38 ALPHA, p38 MAP kinase, p38 MAPK, p38 mitogen activated protein kinase, p38alpha Exip, Stress Activated Protein Kinase 2A.
Molecular weight	Calculated MW: 41 kDa; Observed MW: 41 kDa
Uniprot ID	Q16539
Gene ID	1432
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-p38 Rabbit mAb [49C01B92] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Background

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

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R013794 Anti-p38 Rabbit mAb [49C01B92]

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