Western Blot - Anti-Histone H3 Rabbit mAb [42I15C31]

All lanes: R013738 at 1:2,000 dilution

Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

Lane 2: HepG2 (Human hepatocarcinoma epithelial cell) whole cell lysates

Lane 3: HCT116 (Human colorectal carcinoma epithelial cell) whole cell lysates

Lane 4: 293T(Human embryonic kidney cell) whole cell lysates

Lane 5: A431(Human epidermoid teratoma cell line) whole cell lysates

Lane 6: MCF-7(human breast adenocarcinoma epithelial cell) whole cell lysates

Lane 7: U87(Human malignant glioblastoma epithelial cell) whole cell lysates

Lane 8: THP-1(Human monocytic leukemia cell) whole cell lysates

Lane 9: K562(Human chronic myeloid leukemia cell) whole cell lysates

Lane 10: Raw264.7(Mouse mononuclear macrophage leukemia cell) whole cell lysates

Lane 11: Mouse liver whole tissue lysates

Lane 12: PC-12(Rat adrenal pheochromocytoma epithelial cell) whole cell lysates

Lane 13: Rat brain whole tissue lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG(H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 15 kDa

Observed band size: 17 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunohistochemistry - Anti-Histone H3 Rabbit mAb [42I15C31]

Sample: Paraformaldehyde-fixed, paraffin embedded, human lung cancer tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R013738 at 1:100 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP Conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunohistochemistry - Anti-Histone H3 Rabbit mAb [42I15C31]

Sample: Paraformaldehyde-fixed, paraffin embedded, rat colon tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R013738 at 1:100 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP Conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.