Anti-TPP1 Rabbit mAb [62K74M84]

抗体系列

Anti-TPP1 Rabbit mAb [62K74M84]

Purified Recombinant Rabbit Monoclonal Antibody

一键复制产品信息

货号	规格	价格
R013603
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

立即购买

Western Blot - Anti-TPP1 Rabbit mAb [62K74M84]

All lanes: R013603 at 1:1,000 dilution

Lane 1: HepG2 (Human hepatocarcinoma epithelial cell) whole cell lysates

Lane 2: HCT116 (Human colorectal carcinoma epithelial cell) whole cell lysates

Lane 3: 293T (Human embryonic kidney cell) whole cell lysates

Lane 4: K562 (Human chronic myeloid leukemia cell) whole cell lysates

Lane 5: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 61,35 kDa

Observed band size: 61,48 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunohistochemistry - Anti-TPP1 Rabbit mAb [62K74M84]

Sample: Paraformaldehyde-fixed, paraffin embedded human renal carcinoma tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R013603 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunohistochemistry - Anti-TPP1 Rabbit mAb [62K74M84]

Sample: Paraformaldehyde-fixed, paraffin embedded human endometrial carcinoma tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R013603 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

产品信息
说明书
已发表文献

Product Name	Anti-TPP1 Rabbit mAb
Description	Purified Recombinant Rabbit Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P/IF (Tissue-P), ELISA
Dilution	WB 1:1,000~1:2,000; IHC-P 1:100~1:200; IF 1:100~1:200
Reactivity	Human
Host	Rabbit
Clonality	Monoclonal
Clone No.	62K74M84
Isotype	IgG
Label	Unconjugated
Immunogen	Recombinant protein of human TPP1
Format	Affinity purified monoclonal antibody supplied in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

Synonyms	CLN2; GIG1; UNQ267/PRO304; TPP1; Tripeptidyl-peptidase 1; TPP-1; Cell growth-inhibiting gene 1 protein; Lysosomal pepstatin-insensitive protease; Tripeptidyl aminopeptidase; Tripeptidyl-peptidase I; LPIC; TPP-I.
Molecular weight	Calculated MW: 61,35 kDa; Observed MW: 61,48 kDa
Uniprot ID	O14773
Gene ID	1200
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-TPP1 Rabbit mAb [62K74M84] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	This gene encodes a member of the sedolisin family of serine proteases. The protease functions in the lysosome to cleave N-terminal tripeptides from substrates, and has weaker endopeptidase activity. It is synthesized as a catalytically-inactive enzyme which is activated and auto-proteolyzed upon acidification. Mutations in this gene result in late-infantile neuronal ceroid lipofuscinosis, which is associated with the failure to degrade specific neuropeptides and a subunit of ATP synthase in the lysosome. [provided by RefSeq, Jul 2008]

Background

This gene encodes a member of the sedolisin family of serine proteases. The protease functions in the lysosome to cleave N-terminal tripeptides from substrates, and has weaker endopeptidase activity. It is synthesized as a catalytically-inactive enzyme which is activated and auto-proteolyzed upon acidification. Mutations in this gene result in late-infantile neuronal ceroid lipofuscinosis, which is associated with the failure to degrade specific neuropeptides and a subunit of ATP synthase in the lysosome. [provided by RefSeq, Jul 2008]

Cellular Location	Lysosome.Melanosome.Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Tissue Location	Detected in all tissues examined with highest levels in heart and placenta and relatively similar levels in other tissues.

说明书下载

R013603 Anti-TPP1 Rabbit mAb [62K74M84]

总篇数：加载中...

总影响因子：加载中...

最高影响因子：加载中...

加载中...

没有更多数据

产品中心

联系雅酶

上海市闵行区新骏环路760号1号楼3层

info@epizyme.cn

400-058-8030

技术支持(电话/微信)：

张老师 18217238522

罗老师 15021027255

李老师 18301721288

如有任何关于雅酶产品问题
欢迎来信咨询

雅酶微信公众号

抗体

蛋白提取与定量

蛋白电泳

蛋白免疫印迹

蛋白表达纯化与分离

蛋白质

ELISA试剂盒

核酸相关实验

微生物培养

设备与耗材

细胞培养

网站首页