Western Blot - Anti-Junctional Adhesion Molecule 1 Rabbit mAb [15M18L48]

All lanes: R013286 at 1:1,000 dilution

Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates

Lane 2 :HCT116 (Human colorectal carcinoma epithelial cell) whole cell lysates

Lane 3: SCC-9 (Human tongue squamous carcinoma epithelial cell) whole cell lysates

Lane 4: SW620 (Human colorectal carcinoma epithelial cell) whole cell lysates

Lane 5: 293T (Human embryonic kidney cell) whole cell lysates

Lane 6: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG(H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 32 kDa

Observed band size: 35 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunofluorescence - Anti-Junctional Adhesion Molecule 1 Rabbit mAb [15M18L48]

Sample: A431 cells

The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.5% Triton X-100 for 10 minutes and then blocked with 5% BSA in 0.1% PBS-Tween for 0.5 hours.

Primary antibodies: R013286 at 1:100 dilution

Secondary antibodies: Goat anti-Rabbit (488) at 1:1,000 dilution (shown in green)

Nuclei were stained with DAPI (shown in blue).