Anti-Phospho-p53 (Ser6) Rabbit mAb [62K64K10]

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Anti-Phospho-p53 (Ser6) Rabbit mAb [62K64K10]

Purified Recombinant Rabbit Monoclonal Antibody

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货号	规格	价格
R013163
	20µL	¥588.00
	50µL	¥1080.00
	100µL	¥1780.00

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Western Blot - Anti-Phospho-p53 (Ser6) Rabbit mAb [62K64K10]

All lanes: R013163 at 1:1,000 dilution

Lane 1: HepG2 (Human hepatocarcinoma epithelial cell) whole cell lysates

Lane 2: HCT116 (Human colorectal carcinoma epithelial cell) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP Conjugated (Cat. No. LF102) at 1:5,000 dilution

Predicted band size: 44 kDa

Observed band size: 53 kDa

Developed using the ECL technique (Cat. No. SQ201).

Immunohistochemistry - Anti-Phospho-p53 (Ser6) Rabbit mAb [62K64K10]

Sample: Paraformaldehyde-fixed, paraffin embedded human ovarian cancer tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R013163 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Immunohistochemistry - Anti-Phospho-p53 (Ser6) Rabbit mAb [62K64K10]

Sample: Paraformaldehyde-fixed, paraffin embedded human breast cancer tissue

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 30 mins.

Primary antibody: R013163 at 1:200 dilution

Secondary antibody: Goat Anti-Rabbit IgG (H+L), HRP conjugated at 1:1,000 dilution

DAB was used as the chromogen.

Counter stained with hematoxylin.

Positive/negative staining were presented.

Only the secondary antibody was used as the negative control.

Product Name	Anti-Phospho-p53 (Ser6) Rabbit mAb
Description	Purified Recombinant Rabbit Monoclonal Antibody
Application Applications Legend: WB=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	WB, IHC-P/IF (Tissue-P), ELISA
Dilution	WB 1:1,000~1:2,000; IHC-P 1:100~1:200; IF 1:100~1:200
Reactivity	Human
Host	Rabbit
Clonality	Monoclonal
Clone No.	62K64K10
Isotype	IgG
Label	Unconjugated
Immunogen	A synthetic phosphopeptide corresponding to residues surrounding Ser6 of human p53
Format	Affinity purified monoclonal antibody supplied in PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.

Synonyms	TP53 \| p53 (p-S33); p-p53; phospho-p53; BCC7; BMFS5; LFS1; P53; TRP53; Tp53; bbl; bfy; bhy; p44; P53_BOVIN; Tumor suppressor p53; P53_HUMAN; Antigen NY-CO-13; Phosphoprotein p53; P53_MOUSE; P53_RAT.
Molecular weight	Calculated MW: 44 kDa; Observed MW: 53 kDa
Uniprot ID	P04637
Gene ID	7157
Storage	Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
Precautions	Anti-Phospho-p53 (Ser6) Rabbit mAb [62K64K10] is for research use only and not for use in diagnostic or therapeutic procedures.

Background	This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mice deficient for this gene are developmentally normal but are susceptible to spontaneous tumors. Evidence to date shows that this gene contains one promoter, in contrast to alternative promoters of the human gene, and transcribes a few of splice variants which encode different isoforms, although the biological validity or the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]

Background

This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mice deficient for this gene are developmentally normal but are susceptible to spontaneous tumors. Evidence to date shows that this gene contains one promoter, in contrast to alternative promoters of the human gene, and transcribes a few of splice variants which encode different isoforms, although the biological validity or the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]

Cellular Location	Cytoplasm Nucleus Nucleus PML body Endoplasmic reticulum Mitochondrion matrix Cytoplasm Cytoskeleton Microtubule organizing center Centrosome Recruited into PML bodies together with CHEK2 (PubMed:12810724). Translocates to mitochondria upon oxidative stress (PubMed:22726440). Translocates to mitochondria in response to mitomycin C treatment (PubMed:27323408). Competitive inhibition of TP53 interaction with HSPA9/MOT-2 by UBXN2A results in increased protein abundance and subsequent translocation of TP53 to the nucleus (PubMed:24625977). Isoform 1 Nucleus Cytoplasm Predominantly nuclear but localizes to the cytoplasm when expressed with isoform 4. Isoform 2 Nucleus Cytoplasm Localized mainly in the nucleus with minor staining in the cytoplasm. Isoform 3 Nucleus Cytoplasm Localized in the nucleus in most cells but found in the cytoplasm in some cells. Isoform 4 Nucleus Cytoplasm Predominantly nuclear but translocates to the cytoplasm following cell stress. Isoform 7 Nucleus Cytoplasm Localized mainly in the nucleus with minor staining in the cytoplasm. Isoform 8 Nucleus Cytoplasm Localized in both nucleus and cytoplasm in most cells. In some cells, forms foci in the nucleus that are different from nucleoli. Isoform 9 Cytoplasm

Cellular Location

Cytoplasm Nucleus Nucleus PML body Endoplasmic reticulum Mitochondrion matrix Cytoplasm Cytoskeleton Microtubule organizing center Centrosome Recruited into PML bodies together with CHEK2 (PubMed:12810724). Translocates to mitochondria upon oxidative stress (PubMed:22726440). Translocates to mitochondria in response to mitomycin C treatment (PubMed:27323408). Competitive inhibition of TP53 interaction with HSPA9/MOT-2 by UBXN2A results in increased protein abundance and subsequent translocation of TP53 to the nucleus (PubMed:24625977). Isoform 1 Nucleus Cytoplasm Predominantly nuclear but localizes to the cytoplasm when expressed with isoform 4. Isoform 2 Nucleus Cytoplasm Localized mainly in the nucleus with minor staining in the cytoplasm. Isoform 3 Nucleus Cytoplasm Localized in the nucleus in most cells but found in the cytoplasm in some cells. Isoform 4 Nucleus Cytoplasm Predominantly nuclear but translocates to the cytoplasm following cell stress. Isoform 7 Nucleus Cytoplasm Localized mainly in the nucleus with minor staining in the cytoplasm. Isoform 8 Nucleus Cytoplasm Localized in both nucleus and cytoplasm in most cells. In some cells, forms foci in the nucleus that are different from nucleoli. Isoform 9 Cytoplasm

Tissue Location	Ubiquitous. Isoforms are expressed in a wide range of normal tissues but in a tissue-dependent manner. Isoform 2 is expressed in most normal tissues but is not detected in brain, lung, prostate, muscle, fetal brain, spinal cord and fetal liver. Isoform 3 is expressed in most normal tissues but is not detected in lung, spleen, testis, fetal brain, spinal cord and fetal liver. Isoform 7 is expressed in most normal tissues but is not detected in prostate, uterus, skeletal muscle and breast. Isoform 8 is detected only in colon, bone marrow, testis, fetal brain and intestine. Isoform 9 is expressed in most normal tissues but is not detected in brain, heart, lung, fetal liver, salivary gland, breast or intestine.

Tissue Location

Ubiquitous. Isoforms are expressed in a wide range of normal tissues but in a tissue-dependent manner. Isoform 2 is expressed in most normal tissues but is not detected in brain, lung, prostate, muscle, fetal brain, spinal cord and fetal liver. Isoform 3 is expressed in most normal tissues but is not detected in lung, spleen, testis, fetal brain, spinal cord and fetal liver. Isoform 7 is expressed in most normal tissues but is not detected in prostate, uterus, skeletal muscle and breast. Isoform 8 is detected only in colon, bone marrow, testis, fetal brain and intestine. Isoform 9 is expressed in most normal tissues but is not detected in brain, heart, lung, fetal liver, salivary gland, breast or intestine.

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R013163 Anti-Phospho-p53 (Ser6) Rabbit mAb [62K64K10]

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