Purified Rabbit Monoclonal Antibody
| 货号 | 规格 | 价格 |
|---|---|---|
| R013033 | ||
| 20µL | ¥588.00 | |
| 50µL | ¥1080.00 | |
| 100µL | ¥1780.00 |
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| Product Name | Anti-ATG4A Rabbit mAb |
|---|---|
| Description | Purified Rabbit Monoclonal Antibody |
Application
|
WB, IHC-P/IF (Tissue-P), IP, ELISA |
| Dilution | WB 1:500~1:1,000; IHC-P 1:50~1:100; IP 1:20 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | 22M75L07 |
| Isotype | IgG |
| Label | Unconjugated |
| Immunogen | A synthetic peptide of human ATG4A |
| Format | Buffer System: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA Purification: Affinity Purified. |
| Synonyms | ATG4A, APG4A, AUTL2, Cysteine protease ATG4A, AUT-like 2 cysteine endopeptidase, Autophagin-2, Autophagy-related cysteine endopeptidase 2, Autophagy-related protein 4 homolog A, hAPG4A. |
|---|---|
| Molecular weight | Calculated MW: 45 kDa; Observed MW: 45 kDa |
| Uniprot ID | Q8WYN0 |
| Gene ID | 115201 |
| Storage | Shipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage. |
| Precautions | Anti-ATG4A antibody [22M75L07] is for research use only and not for use in diagnostic or therapeutic procedures. |
| Background | The cysteine protease Atg4 is pivotal to autophagosome membrane generation and regulation. Atg4 primes the Atg8 homologue for lipidation by cleaving its carboxy terminus and exposing its glycine residue for E1-like enzyme Atg7. The Atg8 homologue is transferred to the E2-like enzyme Atg3 before forming the Atg8-PE conjugate. During later stages of autophagy, Atg4 can reverse this lipidation event by cleaving PE, thereby recycling the Atg8 homologue. |
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