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Anti-ATG4A Rabbit mAb [22M75L07]

Purified Rabbit Monoclonal Antibody

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货号 规格 价格
R013033
20µL ¥588.00
50µL ¥1080.00
100µL ¥1780.00

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Anti-ATG4A Rabbit mAb [22M75L07]
Product NameAnti-ATG4A Rabbit mAb
DescriptionPurified Rabbit Monoclonal Antibody
Application
  • Applications Legend:
  • WB=Western Blotting
  • IHC-P=Immunohistochemistry (Paraffin)
  • IHC-F=Immunohistochemistry (Frozen)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P/IF (Tissue-P), IP, ELISA
DilutionWB 1:500~1:1,000; IHC-P 1:50~1:100; IP 1:20
ReactivityHuman
HostRabbit
ClonalityMonoclonal
Clone No.22M75L07
IsotypeIgG
LabelUnconjugated
ImmunogenA synthetic peptide of human ATG4A
FormatBuffer System: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% BSA Purification: Affinity Purified.
SynonymsATG4A, APG4A, AUTL2, Cysteine protease ATG4A, AUT-like 2 cysteine endopeptidase, Autophagin-2, Autophagy-related cysteine endopeptidase 2, Autophagy-related protein 4 homolog A, hAPG4A.
Molecular weightCalculated MW: 45 kDa; Observed MW: 45 kDa
Uniprot ID Q8WYN0
Gene ID 115201
StorageShipped on wet ice. Store at -20℃. Stable for 24 months from date of receipt. Aliquoting is unnecessary for -20℃ storage.
PrecautionsAnti-ATG4A antibody [22M75L07] is for research use only and not for use in diagnostic or therapeutic procedures.
Background The cysteine protease Atg4 is pivotal to autophagosome membrane generation and regulation. Atg4 primes the Atg8 homologue for lipidation by cleaving its carboxy terminus and exposing its glycine residue for E1-like enzyme Atg7. The Atg8 homologue is transferred to the E2-like enzyme Atg3 before forming the Atg8-PE conjugate. During later stages of autophagy, Atg4 can reverse this lipidation event by cleaving PE, thereby recycling the Atg8 homologue.

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